Organelle and translocatable forms of glyoxysomal malate dehydrogenase
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چکیده
منابع مشابه
Organelle and translocatable forms of glyoxysomal malate dehydrogenase
doi:10.1111/j.1742-4658.2004.04475.x Many organelle enzymes coded for by nuclear genes have N-terminal sequences, which directs them into the organelle (precursor) and are removed upon import (mature). The experiments described below characterize the differences between the precursor and mature forms of watermelon glyoxysomal malate dehydrogenase. Using recombinant protein methods, the precurso...
متن کاملOrganelle and translocatable forms of glyoxysomal malate dehydrogenase. The effect of the N-terminal presequence.
Many organelle enzymes coded for by nuclear genes have N-terminal sequences, which directs them into the organelle (precursor) and are removed upon import (mature). The experiments described below characterize the differences between the precursor and mature forms of watermelon glyoxysomal malate dehydrogenase. Using recombinant protein methods, the precursor (p-gMDH) and mature (gMDH) forms we...
متن کاملA cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein.
A plant cysteine endopeptidase with a molecular mass of 35 kD was purified from microbodies of germinating castor bean (Ricinus communis) endosperm by virtue of its capacity to specifically process the glyoxysomal malate dehydrogenase precursor protein to the mature subunit in vitro. Processing of the glyoxysomal malate dehydrogenase precursor occurs sequentially in three steps, the first inter...
متن کاملGlyoxysomal malate dehydrogenase from watermelon is synthesized with an amino-terminal transit peptide.
The isolation and sequence of a cDNA clone encoding the complete glyoxysomal malate dehydrogenase [gMDH; (S)-malate:NAD+ oxidoreductase, EC 1.1.1.37] of watermelon cotyledons are presented. Partial cDNA clones were synthesized in a three part strategy, taking advantage of the polymerase chain reaction technology with oligonucleotides based on directly determined amino acid sequences. Subsequent...
متن کاملMultiple forms of supernatant malate dehydrogenase in salmonid fishes.
Salmon generally possess three forms of supernatant malate dehydrogenase whose subunit compositions are indicated by the formulas AA, AB, and BB. Each of the homodimers (AA and BB) has now been purified to homogeneity. The two enzymes are similar in molecular size and in catalytic properties to the supernatant malate dehydrogenases of higher vertebrates; both are catalytically distinct from the...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2005
ISSN: 1742-464X,1742-4658
DOI: 10.1111/j.1742-4658.2004.04475.x